Compared with the in vitro cell model, the in vitro 3D model in the stroke environment is closer to the human stroke environment and can meet the purpose of disease modeling and high-throughput screening. Therefore, the in vitro 3D model has unique advantages in stroke research. In addition, it was found that 3D brain-like organs derived from human pluripotent stem cells overcame the limitations of stem cell - based transplantation therapy to a certain extent, and showed many advantages, including abundant cell types, wide cell sources, a large number of cells, controllable cell differentiation and a certain tissue volume. 

Brain Organoid Modeling

There are two methods of brain organoid culture based on the orientation of differentiation. One is the organoid structure formed by stem cells during differentiation, which is independent of exogenous morphogenetic factors and contains multiple brain regions. The other is to add exogenous morphogenetic factors and neurotrophic factors at a specific time according to the regulatory mechanism of the human brain development process, so as to cultivate specific brain organoids. Ace Neuroscience has developed sophisticated solutions for both approaches, making it possible to develop brain organ models suitable for stroke research to advance the understanding of stroke pathologies and develop stroke therapies.

Other 3D In Vitro Brain Modeling

In addition to brain organoids, neurospheres, neural aggregates, neural rosette, and cortical spheres are also widely used as 3D models of the brain for the study of various neurological diseases. They all mimic brain pathology to varying degrees and, therefore, can be used for high-throughput screening. Ace Neuroscience provides relevant model construction services to promote the development of stroke pathology mechanisms and stroke therapies in a comprehensive manner.

• Neurospheres are widely used to identify neural stem cells in the central nervous system
• The neural aggregates form an embryoid body for subsequent studies. It often represents the early stages of embryonic development
• Salient features of the neural rosette nerve induction protocol, which mimics the early neurogenesis process
• The cortical sphere can show the unique self-organization of the human neocortex.