Typically, target DNA is amplified to a certain level by employing PCR for DNA amplification, followed by quantitative detection, to detect tiny amounts of nucleic acid. The most traditional method of DNA amplification is the polymerase chain reaction (PCR), which is typically used in laboratory-scale PCR cycles for a variety of purposes, including diagnosis, cloning, and sequencing. These days, it is possible to miniaturize the procedure in microfluidic chips, cutting the expense of producing and using biological materials as well as the length of time it takes for DNA amplification. Additionally, microfluidic chip-based devices can be easily integrated with additional DNA processing and analysis procedures.
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